PRF Techniques

Gold Coast Oral & Maxillofacial Surgery Study Club

Study Club Reference Guide

Your Kit Contents

Everything you need to prepare and apply PRF in your clinical setting — from centrifuge to hand instruments.

PRF Centrifuge

Preset settings for A-PRF and i-PRF. Good results staying on A-PRF settings for both.

Tubes

24× S-PRF (green) for sticky bone. 100× A-PRF (red) for plugs and membranes.

Vacutainers

35× 21G Butterflies. Smaller diameter needles are less painful but more destructive to blood cells.

Hand Instruments

Rat-tooth forceps, graft scoop, graft packer, and transportation pad.

PRF Centrifuge

Preset Programs

The centrifuge has preset ideal settings for both A-PRF (plug and membrane) and i-PRF (sticky bone). We have found good results staying on A-PRF settings for both applications.

Tubes

S-PRF — Green Tubes

24× 10ml tubes. Used for producing i-PRF for sticky bone preparation.

A-PRF — Red Tubes

100× 10ml tubes. Produce PRF plugs which can also be flattened into membranes.

Vacutainers & Tube Rack

35× 21G Butterflies

Smaller diameter needles (22G and 23G) are less painful but more destructive to blood cells. 21G is the recommended gauge.

Tube Rack

Used to keep centrifuged tubes upright after spinning to preserve the separated layers before use.

PRF Press Box

Used to compress PRF plugs into flat membranes for use in GBR and other applications.

PRF Trays & Hand Instruments

PRF Trays

Flat tray for flat membranes. Rounded tray for sticky bone mixing.

Hand Instruments

  • Rat-tooth forceps — for removing the slug from the tube
  • Graft scoop
  • Graft packer
  • Transportation pad

PomPac Cooler

Why Use the PomPac?

A colder tube will produce more PRF. The PomPac can be kept in the freezer and tubes can be cooled inside it prior to blood draw, maximizing PRF yield.

The PRF Plug — Socket Preservation

Draw 1–2 A-PRF tubes per socket, remembering to spin counterbalanced. Form the plug and deliver it into the socket.

The PRF Plug — Socket Preservation

Draw 1–2 A-PRF tubes per socket, remembering to spin counterbalanced. Form the plug and deliver it into the socket.

PRF Plug — Clinical Evidence

"The results showed an acceptable efficacy for PRF without graft materials in alveolar ridge preservation. This material is cost-effective and could be easily prepared. PRF application in extraction sockets yielded similar results to FDBA."

Azangookhiavi et al. — Comparison of the Efficacy of PRF and Bone Allograft for Alveolar Ridge Preservation after Tooth Extraction. PMCID: PMC7882193

Socket Preservation — Histomorphometric Study

A randomized controlled trial of 90 patients compared FDBA+PRF membrane, PRF alone, and a control group. Implants were placed 4 months post-extraction with bone biopsies analyzed for vital bone and connective tissue.

60.79%

PRF Alone

Vital bone formation (±9.72%)

63.29%

FDBA + PRF Membrane

Vital bone formation (±13.03%)

90

Patients

Randomized across 3 groups

Ivanova et al. — Both test groups showed significantly higher vital bone formation vs. control. No statistically significant difference between PRF alone and FDBA+PRF. PMID: 34299902

A-PRF vs. FDBA — Randomized Controlled Trial

40 patients requiring extraction and implant replacement were randomized into four ridge preservation groups: A-PRF, A-PRF+FDBA, FDBA, or blood clot. A-PRF was prepared at 1,300 rpm for 8 minutes. Bone cores were harvested after ~15 weeks.

Ridge Height Loss

Blood clot: 3.8 ± 2.0 mm vs. A-PRF: 1.8 ± 2.1 mm vs. A-PRF+FDBA: 1.0 ± 2.3 mm (p < 0.05)

Vital Bone

A-PRF: 46% ± 18% vs. FDBA: 29% ± 14% (p < 0.05) — significantly more vital bone with A-PRF

Bone Mineral Density

FDBA: 551 ± 58 mg/cm³ vs. blood clot: 487 ± 64 mg/cm³ (p < 0.05)

Clark et al. — Advanced PRF and FDBA for ridge preservation. PMID: 29683498

The PRF Plug — Wisdom Tooth Sockets

Pain & Analgesia

Significantly decreases post-op pain and reduces analgesic intake on days 3 and 7.

Edema & Trismus

Reduces post-operative edema and trismus, with statistically significant results at day 3 and 7 (p < 0.05).

Soft Tissue Healing

Improved soft tissue healing and reduced dry socket risk. Reduced hematomas and skin warmth on day 3.

Bone Density

Increased bone density in the socket. A-PRF was more important than tooth position or procedure duration in reducing complications.

Wisdom Tooth Study — Key Findings

A study of 100 patients undergoing lower third molar odontectomy (Gdańsk, 2018–2019) compared 50 patients with immediate A-PRF socket filling vs. 50 controls. Clinical features were assessed on days 3, 7, and 14.

The PRF Plug — Indirect Sinus Lift

If there was a way to lift the membrane and leave it lifted without graft — it would fill with bone. Using bone graft particles carries risk: what do you do if it migrates into the sinus? The PRF plug offers a safer alternative.

The PRF Plug — Direct Sinus Lift

PRF as the sole filling material during lateral sinus lift with immediate implantation. Study by Mazor et al. — 25 sinus elevations on 20 patients, 41 implants placed in residual bone height of 1.5–6 mm (mean 2.9 ± 0.9 mm).

10.1mm

Mean Bone Gain

Range: 7–13 mm (±0.9 mm)

41

Implants Placed

Zero implants lost at 6 months

25

Sinus Elevations

All biopsies showed well-organized vital bone

"PRF as the sole filling material stabilized a high volume of natural regenerated bone in the sub-sinus cavity up to the tip of the implants. A simple and inexpensive biomaterial." — Mazor et al., J Periodontol 2009

Direct Sinus Lift — Surgical Views

Direct Sinus Lift — Intraoperative & CBCT

Intraoperative view (F): PRF plug inserted into the sinus cavity.

CBCT (G): Pre- and post-operative sagittal views showing sinus floor elevation.

Direct Sinus Lift — Pre & Post CBCT

Pre-operative (A): Right maxillary sinus. Sinus floor at approximately the 30mm mark.

Intraoperative (B): Implant placed with PRF plug visible in the sinus cavity.

Post-operative (D): Sinus floor now at ~40mm — successful elevation confirmed.

Creating the PRF Membrane

PRF Membrane — Plugs to Membranes

PRF plugs prior to pressing — yellowish fibrin matrix with reddish base.

Resulting PRF membranes after pressing — flattened, rich in platelets and growth factors.

PRF Membrane — GBR Application

PRF membranes can be placed over collagen membranes to improve tissue healing efficiency in Guided Bone Regeneration procedures.

PRF Membrane — Implants (Poncho Technique)

Use the poncho technique to support your implant tissue results. The PRF membrane is draped over the implant and surrounding gingiva to promote healing.

PRF membrane being handled with aspiration instrument on perforated surface.

PRF membrane placed over implant to support tissue healing.

Sticky Bone — Preparation Protocol

Sticky Bone — Step by Step

A: Draw S-PRF from green tube into syringe over bone graft.

B: Pour S-PRF into bowl and mix with bone graft.

C: Mixed S-PRF and graft — reddish color from fibrin.

D: Sticky bone formed — graft coated in fibrin matrix.

Full Clinical Workflow

From S-PRF draw (A) through graft mixing (B–D), PRF plug preparation (E), sticky bone application (F), and intraoral delivery (G–H) — the complete PRF workflow from bench to chairside.

PRF Techniques — Quick Reference Summary

1

PRF Plug

1–2 A-PRF (red) tubes per socket. Spin counterbalanced. Deliver plug directly into socket or sinus.

2

PRF Membrane

Centrifuge A-PRF plug, then press in PRF press box. Use for GBR overlay or implant poncho technique.

3

Sticky Bone

Draw S-PRF (green tube). Mix with bone graft of choice. Wait 3–5 minutes for fibrin matrix to form.

4

PomPac Tip

Pre-cool tubes in the PomPac freezer pouch. Colder tubes produce more PRF — maximize your yield.

Dr. Gregory Yunov

Diplomate, American Board of Oral and Maxillofacial Surgery

Dr. Yunov is a highly experienced oral and maxillofacial surgeon, dedicated to providing advanced care and exceptional patient outcomes at Gold Coast Oral Surgery.

Gold Coast Oral Surgery

Phone: 718-483-1637

Website: goldcoastoralsurgery.com

Email: dryunov@goldcoastoms.com

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